Ấn phẩm:
XÁC ĐỊNH LOÀI XÂM LẤN SÂU KEO MÙA THU Spodoptera frugiperda (J.E. Smith) (Lepidoptera : Noctuidae) TRÊN CÂY NGÔ TẠI HÀ NỘI VỤ XUÂN NĂM 2019 [Tài nguyên điện tử]
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The major aim of this study is to identify the identity of an invasive insect observed recently on maize in Viet Nam. The larvae individuals were collected on maize plants grown in Gia Lam - Hanoi in spring 2019 and then reared in laboratory. The identification was done by analyses of the symptoms, the morphological characteristics of developmental phases and adults of 10 male/female pairs and the sequence of the mitochondria cytochrome oxidase subunit I (COI) gene of 2 pupae samples. The symptoms and morphological characteristics of this species were matched perfectly with those of Fall armyworm (FAW) Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae). The BLAST search indicated the COI sequences of the 2 samples were 100% identical with that of FAW available in the GenBank. The COI sequences of the 2 samples were 99.8 -100 % and 98.3 - 98.6 % sequence identical with those of the R (Rice) C (corn) strains of FAW, respectively. Similarly, the COI sequences of the 2 samples contained SacI and AciI sites typical to R (Rice). In the COI tree, the 2 samples were grouped tightly within the R strain cluster of FAW. The COI gene analyses and in comparison with the published information of the genetic structure of FAW suggested that the FAW samples collected in Hanoi would be interstrain hybrids with RC genotype between R mother and C father similar to that in Africa
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2019
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