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Molecular cloning : a laboratory manual. Volume 3
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Tóm tắt
Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. They show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. This book is the indispensable molecular biology laboratory manual and reference source.
Mô tả
Rev. ed. of: Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell. 2001
Năm xuất bản
2012
Tác giả
Green, Michael R.
Nhà xuất bản
Cold Spring Harbor Laboratory Press
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